Purification of a constitutive chitosanase produced byBacillussp. MET 1299 with cloning and expression of the gene
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چکیده
منابع مشابه
Purification of a constitutive chitosanase produced by Bacillus sp. MET 1299 with cloning and expression of the gene.
A chitosanase produced constitutively by Bacillus sp. MET 1299 was purified by SP-Sephadex column chromatography. The molecular weight was estimated to be 52 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Optimal enzyme activity was observed at a pH of 5.5 and temperature of 60 degrees C. The purified chitosanase showed high activity on 90% deacetylated colloidal c...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2004
ISSN: 0378-1097,1574-6968
DOI: 10.1016/j.femsle.2004.09.006